Microtubule shuttles on kinesin-coated glass micro-wire tracks
نویسندگان
چکیده
منابع مشابه
Microtubule shuttles on kinesin-coated glass micro-wire tracks.
Gliding of microtubule filaments on surfaces coated with the motor protein kinesin has potential applications for nano-scale devices. The ability to guide the gliding direction in three dimensions allows the fabrication of tracks of arbitrary geometry in space. Here, we achieve this by using kinesin-coated glass wires of micrometer diameter range. Unlike previous methods in which the guiding tr...
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Nature has evolved specialized molecules, motor proteins, to actively transport molecules over long distances. Such molecule-based energy dependent transport processes would be useful in nanotechnology or material sciences, for example to facilitate local assembly or repair processes, or to mediate rapid transport of nonbiological molecules. In an effort to elucidate how to control the movement...
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The assembly and maintenance of an extended intermediate filament (IF) network in fibroblasts requires microtubule (MT) integrity. Using a green fluorescent protein-vimentin construct, and spreading BHK-21 cells as a model system to study IF-MT interactions, we have discovered a novel mechanism involved in the assembly of the vimentin IF cytoskeleton. This entails the rapid, discontinuous, and ...
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Nopp140 is a nucleolar phosphoprotein of 140 kd that we originally identified and purified as a nuclear localization signal (NLS)-binding protein. Molecular characterization revealed a 10-fold repeated motif of highly conserved acidic serine clusters that contain an abundance of phosphorylation consensus sites for casein kinase II (CK II). Indeed, Nopp140 is one of the most phosphorylated prote...
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An alternative method of micro/nano-transport has been achieved by using motor proteins. Microtubules on a kinesin-coated surface have potential to act as a nano-transport system. When microtubules are used as carriers, either cargo or cargo linkers are attached on the microtubule surface. Such cargo attachments can significantly affect kinesin motion. To deal with the difficulty caused by mole...
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ژورنال
عنوان ژورنال: Biomedical Microdevices
سال: 2014
ISSN: 1387-2176,1572-8781
DOI: 10.1007/s10544-014-9852-6